Proteins are formed from a number of amino acids joined together by dehydration condensation. Amino acids are the basic components of proteins, and their sequence information is the primary structure of proteins. The primary structure of a protein determines the other higher structures of the protein and defines its function. Therefore, protein sequence analysis is helpful for the analysis of protein structure and function.
Protein sequence analysis techniques can be divided into mass spectrometry and non - mass spectrometry. Among them, mass spectrometry can be used for the N-terminal or C-terminal sequencing of proteins, and can also be used for the complete sequencing, de novo sequencing and mutation analysis of proteins. The most famous non-mass spectrometry method is the Edman degradation method, which is mainly used for N-terminal sequencing of proteins.
